Mass of genetic material composed of DNA and proteins that condense to form chromosomes during eukaryotic cell division. Chromatin is located in the nucleus of a cell. During prophase of mitosis, chromatin fibers become coiled into chromosomes with each chromosome having two chromatids joined at a centromere. A complex of nucleic acids (e.g. DNA or RNA) and proteins (histones), which condenses to form a chromosome during cell division. In eukaryotic cells, it is found within the cell nucleus whereas in prokaryotic cells, it is found within the nucleoid. Its functions are to package DNA into a smaller volume to fit in the cell, strengthen the DNA to allow mitosis and meiosis, and to serve as a mechanism to control expression.
Chromatin is a complex of DNA and protein found inside the nuclei of eukaryotic cells. The nucleic acids are generally in the form of double-stranded DNA (a double helix). The major proteins involved in chromatin are histone proteins, but other chromosomal proteins are prominent too. DNA is packaged into chromatin thereby constraining the size of the molecule and allowing the cell to control expression of the chromatin-packaged genes. Changes in chromatin structure are affected mainly by methylation (DNA and proteins) and acetylation (proteins). Chromatin structure is also relevant to DNA replication and DNA repair.
Chromatin can be made visible by staining, hence its name, which literally means coloured material.
Levels of DNA condensation.
(1) DNA double-strand helix. (2) Chromatin strand (DNA with histones).
(3) Condensed chromatin during interphase with centromere.
(4) Condensed chromatin during prophase. (Two copies of the DNA molecule are now present)
(5) Chromosome during metaphase.Simplistically, there are three major levels of chromatin organization.