Is this a good explanation of the DNA replication?

Question

The origin of Replication is a special site where the DNA replication begins Multiple enzymes called DNA helicase attach to the DNA and start separating two strands and open up a replication bubble. DNA replication proceeds out in both directions from the replication bubble. At each end of the replication bubble is a Y-shaped region called the replication Fork. The DNA Helix is separated and the synthesizing of new DNA strands begin. The enzyme DNA polymerase helps to speed this reaction up, by adding nucleotides the separated parent strands. Most DNA polymerases need a primer and a DNA template (parental strand) to work. The Leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. Also the Leading strand is synthesized continuously, whereas the lagging strand is synthesized in short fragment s(discontinuously) that are ultimately stitched together. Which are okazaki fragments. DNA ligase helps restore/create phosphodiester bonds and in turn join the sugar phosphate backbone and completing the new DNA strand.


Am i missing anything?

Answer 1

you are missing things, but it depends on the detail you learned



one thing i would say is that DNA polymerase doesnt speed up the reaction, it is responsible for it, without DNA polymerase it couldnt occur



you could also specify which DNA polymerase was responsible



if you learned about DNA gyrase (tension releiving protein) mention it

if you learned about ssbs (single stranded binding proteins) mention it

you sould also mention that the new strands contain half new and half old DNA

you could mention that the DNA polymerase reaches a termination point that ends it

you could mention that multiple replication bubbles occur all across the lenth of the DNA to decrease the time it takes



Like i said it depends onthe detail you learned it in.. but all in all that was a concise and accurate explanation

Answer 2

this is what my professor said i am gonna put it in steps



1.DNA double helix unwinds to form 2 templates *Enzyme needed: DNA helicase

2. Free nucleotides in nucleus bind to their complementary pairs on each template *Enzyme needed: DNA polymerase

3. Newly formed base pairs are spliced together *Enzyme needed: DNA ligase

4. Process continues until entire original DNA molecule is unwind, and two new DNA molecules are produced..



these are my notes from my college lecture about the steps of DNA replication Phase S2 in interphase :)

hope it helps!

Answer 3

hmmm.. the leading strand is called leading because it is 1st to finish without having any problem... so it works on 5'3' then the strand goes downwards opposite to its strand.. it must be 3'5'... while the lagging strand is the 2nd strand that should go downwards opposite to the 2nd strand.. it will be working on the strand 3'5'. so the strand would be created as 5'3' with fragments which aren't completed.. thats why it makes okazaki fragments! there is only one helicase!